Non-destructive DNA extraction and metabarcoding of arthropod bulk samples: a step-by-step protocol

Erscheinungsjahr:

2024

Vollständiger Titel:

Non-destructive DNA extraction and metabarcoding of arthropod bulk samples: a step-by-step protocol

ZFMK-Autorinnen / ZFMK-Autoren:

Dr. Vera Zizka , Dr. Kathrin Langen, Dr. Ameli Kirse, Alice Scherges, Dr. Sarah Bourlat

Org. Einordnung:

Naturschutzökologie, Metabarcoding

Publiziert in:

Pensoft Advanced Books

Publikationstyp:

Buchkapitel

DOI Name:

https://doi.org/10.3897/ab.e119534

Keywords:

DNA isolation, lysis buffer, fixative, sample integrity, non-destructive, metabarcoding, amplicon library preparation

Bibliographische Angaben:

Zizka, V.M.A., Langen, K., Kirse, A., Scherges, A.M., Bourlat, S.J. (2024) Non-destructive DNA extraction and metabarcoding of arthropod bulk samples: a step-by-step protocol. In: Wägele, J.W., Tschan, G.F. (Eds) Weather stations for biodiversity: a comprehensive approach to an automated and modular monitoring system. Advanced Books, Pensoft, 87-102. https://doi.org/10.3897/ab.e119534

Abstract:

In the AMMOD project, flying insects are collected using Malaise traps equipped with automated bottle changers (see Chapter 5). Insect catches are identified using DNA metabarcoding, a promising tool for biodiversity assessments, especially targeting highly diverse groups such as the arthropods. Non-destructive DNA isolation methods are highly desirable for the preservation of sample integrity and subsequent morphological analysis of specimens. In this chapter, we present a comprehensive step-by-step laboratory protocol for the non-destructive DNA extraction of insect bulk samples from lysis buffer followed by all subsequent amplicon library preparation steps required for the sequencing of insect bulk samples on Illumina platforms.